![]() After embedding the brain with Spurr resin, a complete three-dimensional cerebrovascular dataset was acquired using Micro-Optical Sectioning Tomography (MOST). To study the entire macro and micro vascular anatomical structure network, we improved the gelatin-Indian ink vessel perfusion process based on previous work, ,, ,, and obtained a desirable perfused Kunming mouse brain. cerebrovascular connection, has not been achieved yet. However, reconstructing the connections of the blood supply pathway from the feeding vessels to the draining vessels through the capillaries in the whole brain range, i.e. Optical microscopes with three-dimensional scanning and stitching have also been used in preliminary experiments to obtain cerebrovascular dataset –, some resource have been available on the internet ( ). Vascular casts and integrated μCT, SRμ-CT and SEM have been used to image the cerebral vasculature at different resolutions. Several technologies have been developed to attempt to bridge the macro and micro vessels which form the continuous vascular network. In recent years, the study of the vascular network has become a hotspot. This leads to an urgent need for systematic research. On the other hand, microvascular imaging in a small field cannot obtain a clear view of the vessels' origins and destinations.Ĭerebral vessels play significant roles in the development and degradation of the neural network and in the process of maintaining normal brain functions. The existing techniques used to observe the macro vessels cannot visualize the fine branches of the veins, arteries and the capillary network connecting them. However, current cerebrovascular studies focus mainly either on the macro vessels of the whole brain or on the micro vessels in a small local field separately. To observe the smaller, more complex micro vessels, ex vivo two-photon laser scanning microscopy is used to image the capillary network in the cortex to a depth of 1 mm using fluorescent gelatin vessel perfusion. MRI techniques can also visualize the macro vessels in the whole mouse brain. identified and marked the major vessels in the CBA mouse using Microfil perfusion and Micro-CT imaging. The Micro-CT technique, which has been used in mice, can visualize the arterioles and venules in the whole brain. The macro vessels were identified first and were studied with the naked eye. All arteries, veins and capillaries work together to meet the demand for an uninterrupted energy supply for the brain. The topology of the cerebral vasculature, which is the energy transport corridor of the brain, can be used to study cerebral circulatory pathways –. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. National Natural Science Foundation of China. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.įunding: This work was supported by the National Natural Science Foundation of China (Grant Nos. Received: ApAccepted: JanuPublished: January 30, 2014Ĭopyright: © 2014 Xue et al. PLoS ONE 9(1):Įditor: Aernout Luttun, Katholieke Universiteit Leuven, Belgium (2014) Indian-Ink Perfusion Based Method for Reconstructing Continuous Vascular Networks in Whole Mouse Brain. This study provided an effective method for studying the entire macro and micro vascular networks of mouse brain simultaneously.Ĭitation: Xue S, Gong H, Jiang T, Luo W, Meng Y, Liu Q, et al. Besides the observations of fine and complex vascular networks in the reconstructed slices and entire brain views, a representative continuous vascular tracking has been demonstrated in the deep thalamus. The voxel resolution is 0.35×0.4×2.0 µm 3 for the whole brain. With 17 days of work, an integral dataset for the entire cerebral vessels was acquired. Here, we have combined the improved gelatin-Indian ink vessel perfusion process with Micro-Optical Sectioning Tomography for imaging the vessel network of an entire mouse brain. Simultaneous vascular studies of arteries, veins and capillaries have not been achieved in the whole brain of mammals. ![]() Limited by the restrictions of the vascular markers and imaging methods, studies on cerebral vascular structure now mainly focus on either observation of the macro vessels in a whole brain or imaging of the micro vessels in a small region. The topology of the cerebral vasculature, which is the energy transport corridor of the brain, can be used to study cerebral circulatory pathways. ![]()
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